Journal: Nature Communications

Article Title: Intrinsic TGF-β signaling attenuates proximal tubule mitochondrial injury and inflammation in chronic kidney disease

doi: 10.1038/s41467-023-39050-y

Figure Lengend Snippet: a Relative Pgc1α mRNA levels in renal cortices from uninjured and AA injured (6 weeks) mice, measured by RT-qPCR using S12 as housekeeping control gene; n = 4 uninjured, 7 injured (Tgfbr2 fl/fl ) and 4 uninjured, 5 injured (γGT-Cre;Tgfbr2 fl/fl ) mice, uninjured p = 0.05 and injured p = 0.8152. b , c Representative Pgc1α immunohistochemistry (IHC) images and quantification in uninjured and 6 weeks injured renal cortices; n = 4 uninjured, 7 injured (Tgfbr2 fl/fl ) and 4 uninjured, 5 injured (γGT-Cre;Tgfbr2 fl/fl ) mice, uninjured p = 0.0436 and injured p = 0.9983. Scale bars represent 20 μm. d , e Representative endogenous fluorescence images and quantification showing decreased mitophagic flux (mCherry) in γGT-Cre;Tgfbr2 fl/WT ;mito-QC mice compared to Tgfbr2 fl/WT ;mito-QC mice 6 weeks after AA injury; n = 3 (Tgfbr2 fl/WT ;mito-QC) and 4 (γGT-Cre;Tgfbr2 fl/WT ;mito-QC) mice, p = 0.045. Scale bars represent 20 μm. f – h LC3A (I/II) immunoblotting and quantification showing significant decrease of LC3A-I expression in γGT-Cre;Tgfbr2 fl/fl renal cortices 6 weeks after AA injury; n = 5 (Tgfbr2 fl/fl ) and 5 (γGT-Cre;Tgfbr2 fl/fl ) mice, p = 0.0001. Data are presented as mean values ± SEM. Statistical significance was determined by unpaired Student’s t test (two groups) or two-way ANOVA followed by Sidak’s multiple comparisons test, with p < 0.05 considered statistically significant. The dots represent the number of animals per group. * p < 0.05; *** p < 0.001. Source data are provided as a Source data file.

Article Snippet: The following primary antibodies were used: Pink1 (Novus), LC3A (CST), Pgc1α (Merck), cleaved caspase 9 (CST), phospho-Smad3 (CST), total OXPHOS (ab110413, Abcam), Polγ (Santa Cruz), Tom20 (Santa Cruz), α-tubulin (GT114, GeneTex), polyclonal goat anti-rabbit-HRP (31460, Pierce), polyclonal goat anti-mouse-HRP (31430, Pierce).

Techniques: Quantitative RT-PCR, Immunohistochemistry, Fluorescence, Western Blot, Expressing

Journal: Progress in molecular biology and translational science

Article Title: Cellular Metabolism and Aging

doi: 10.1016/bs.pmbts.2017.12.003

Figure Lengend Snippet: SIRT1 activates expression of nuclear encoded mitochondrial genes through deacetylation of PGC1α. The sirtuins SIRT3, SIRT4, and SIRT5 reduce proteotoxic stress by removing protein acetylation in mitochondria (not shown).

Article Snippet: Some of the immediate metabolic changes following NAD + repletion involve enhanced mitochondrial activity through deacetylation and activation of PGC1α by SIRT1, as well as improved mitochondrial proteostasis by SIRT3.

Techniques: Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Methane-Rich Saline: A Potential Resuscitation Fluid for Hemorrhagic Shock

doi: 10.1155/2019/4929107

Figure Lengend Snippet: Sirt3 signaling is involved in MRS-mediated protection during hemorrhagic shock and resuscitation. (a) Protein expression of components of the PGC-1 α /SIRT3/SOD2 signaling pathway (PGC-1 α /SIRT3/SOD2/Ac-SOD2) was detected by western blotting. (b) Relative band intensities (fold of the sham group) are shown. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

Article Snippet: The resulting blots were blocked with 8% skim milk and incubated with anti-Bax antibody (1 : 1000, Sanying Biotechnology, China), anti-Bcl-2 antibody (1 : 1000, Sanying Biotechnology, China), anti-PGC-1 α antibody (1 : 2000, Sanying Biotechnology, China), anti-SIRT3 antibody (1 : 1000, Sanying Biotechnology, China), anti-SOD2 antibody (1 : 1000, Sanying Biotechnology, China), anti-Ac-SOD2 antibody (1 : 1000, Abcam, USK), and anti- β -actin antibody (1 : 10000, Santa Cruz Biotechnology, USA) overnight at 4°C.

Techniques: Expressing, Western Blot